PEI mediated transfection efficiencies and corresponding lentiviral vector titers in CAP-T and adherent HEK293T cells.
Using suspension CAP-T cells from the CAP-GT platform, high titers for HIV-1 based lentiviral vectors are reached, outperforming adherent HEK293T cells.
The CAP cell line produces 5.000-10.000 infectious adenovirus particles per cell. No undesired replication competent adenovirus (RCA) could be detected in 5x10e10 viral particles (VP) from CAP cells, whereas 5-500 RCAs can be detected in 5x10e10 VP produced in HEK293 cells.
CAP-GT cells are highly suitable for high titer production of replication deficient adenoviral vectors.